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Reliability of the testUsing DNA tests to perform paternity testing is today´s most up-to-date way of excluding or confirming, with a high degree of accuracy, a man's paternity to a child. Serologic tests, which started to be abandoned in late 90´s, had significantly lower rates of accuracy and did not provide acceptable results. Oppose to serologic tests, by means of DNA testing, paternity can be confirmed with a very high level of accuracy. Standardly, we use 16 highly polymorphic sites from various regions of genome by means of PCR followed by fragmentation analysis. As soon as we know the types of alleles in tested individuals, we can evaluate which child´s alleles were inherited from the child´s mother (in three-part paternity testing) and if the other allele could be inherited from the father. If the match is found in all tested polymorphisms, there is a biological relationship between the alleged father and the child with a high degree of probability (more than 99,99%). When a two-part paternity testing is applied, the match between alleged father and a child is tested for each polymorphism. If the match is found in all tested polymorphisms, there is a biological relationship between the alleged father and the child with a high degree of probability (more than 99,8%). In some cases in a two-part paternity testing, we use another 17 Y-polymorphisms for a father-son combination (without surcharge), i.e. 33 polymorphisms in total. Reliability of result can be influenced in a case where men, candidate fathers, are genetically closed individuals, that is close relatives. This possibility is discussed in the brochure in the P-TEST. |